This antibody recognises the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1. recognises monocytes, macrophages and neutrophils by flow cytometry and is thought to be against the homologue of human CD11b. In immunohistochemistry good staining of macrophages is observed.
Applications
Flow Cytometry, IHC-Fr, IP, IF
Dilutions
Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood .
Reactivity
Rabbit
Immunogen
Rabbit adherent blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
198
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rabbit CD11b antibody, clone 198 recognizes the rabbit CD11b cell surface glycoprotein, also known as the integrin alpha M chain and MAC-1. Mouse anti Rabbit CD11b antibody, clone 198 immunoprecipitates two proteins of molecular weight 165 kD and 95 kD from granulocytes. It recognizes monocytes, macrophages and neutrophils by flow cytometry and is thought to be against the homologue of human CD11b. In immunohistochemistry good staining of macrophages is observed.