CD11A expression in Human Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-CD11A Antibody (A82902) at 0.5µg/ml and detected by chemiluminescence.
CD11A expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-CD11A Antibody (A82902) at 2µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for CD11A expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
CD11A expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CD11A Antibody (A82902) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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