Anti-CCT2 Antibody (A15271)

Name: Anti-CCT2 Antibody
Brand: Antibodies.com
SKU: A15271
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to CCT2 for WB, ICC/IF, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CCT2 Antibody

Description

Rabbit polyclonal antibody to CCT2.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

MCF7, SKOV3, A-549, HT-29, Mouse spleen, Mouse ovary, Mouse heart.

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse

Immunogen

A recombinant fusion protein corresponding to amino acids 206-535 of human CCT2.

Sequence

GGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

57 kDa

Predicted MW

57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

99D8.1, CCT-beta, CCTB, Chaperonin containing T-complex polypeptide 1 subunit 2, T-complex protein 1 subunit beta, TCP-1-beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CCT2 Antibody (A15271)

CCT2 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-CCT2 Antibody (A15271) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunofluorescence - Anti-CCT2 Antibody (A15271)

CCT2 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-CCT2 Antibody (A15271) followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunoprecipitation - Anti-CCT2 Antibody (A15271)

CCT2 expression in 200 µg extracts of A549 cells analyzed by western blot following immunoprecipitation, both using Anti-CCT2 Antibody (A15271) at 3 µg and a dilution of 1:1000, respectively.

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