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Anti-CCR9 Antibody [9B1] (PE) (A279562)

$535

Rat monoclonal [9B1] antibody to CCR9 (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-CCR9 Antibody [9B1] (PE)
Description
Rat monoclonal [9B1] antibody to CCR9 (PE).
Specificity
This antibody recognises mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9. CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Reactivity
Mouse
Immunogen
A peptide corresponding to 25 amino acids from the N-terminus of murine CCR9, conjugated to Keyhole Limpet Haemocyanin.
Host
Rat
Clonality
Monoclonal
Clone ID
9B1
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD199 antibody, clone 9B1 detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9. CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+ release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.
Synonyms
C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, CDw199, G-protein coupled receptor 28, GPR-9-6, GPR28
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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