Anti-CCR5 Antibody [T21/8] (A281805)

Anti-CCR5 Antibody [T21/8]

Mouse monoclonal [T21/8] antibody to CCR5.

This antibody recognises CD195 also known as C-C chemokine receptor 5 (CCR5) which belongs to the GPCR protein family and plays a role in inflammation by targeting cells to sites of inflammation.CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013).During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions. An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection. This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections.

Flow Cytometry, IP, WB, ELISA, ICC

Human

CCR5 peptide (Met1-Lys22) Keyhole Limpet Haemocyanin conjugate.

Mouse

Monoclonal

T21/8

IgG1

Protein A affinity chromatography of tissue culture supernatant.

>95% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze!

Mouse anti Human CD195, clone T21/8 recognizes CD195 also known as C-C chemokine receptor 5 (CCR5) which belongs to the GPCR protein family and plays a role in inflammation by targeting cells to sites of inflammation. CD195 is expressed in a range of hematopoietic cells including macrophages, dendritic cells and memory T cells. It is also expressed by non-hematopoietic cells including vascular smooth muscle cells and astrocytes (Barmania &Pepper 2013). During inflammation CD195 expression is upregulated in CD8+ cells, allowing the cells to migrate to sites with CD4+ T cell and dendritic cell interactions (Castellino et al. 2006). An additional well characterized feature of CD195 is its role as an essential co-receptor for macrophage tropic HIV strains, allowing the virus to enter the target cell during initial infection (Dragic et al. 1996). This clone T28/1 has been used in flow cytometry experiments to examine expression levels in CD195 overexpressing PM1 cells. These cells were then used to examine the effect of CD195 expression levels on HIV infections (Monde et al. 2007).

C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CD195, CHEMR13, CMKBR5, HIV-1 fusion coreceptor