This antibody recognises the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5.CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 100µl fresh whole blood
Reactivity
Human
Immunogen
CHO cells transfected with human CCR5.
Host
Rat
Clonality
Monoclonal
Clone ID
HEK/1/85a
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human CD195 antibody, clone HEK/1/85a recognizes the human CD195 cell surface antigen, a 45 kDa glycoprotein also known as CCR5.CD195 acts as a receptor for a number of chemokines including RANTES and eotaxin and serves as a co-receptor for the entry of HIV into cells. CD195 is expressed by a subset of T lymphocytes and by monocytes.