Western blot analysis of rat brain cell lysates showing detection of ~41.9 kDa CCR2 protein using Anti-CCR2 Antibody (A305147) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~41.9 kDa.
Immunohistochemistry analysis of human tonsil, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-CCR2 Antibody (A305147) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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