Anti-Cathepsin B Antibody [ARC0395] (A308610)

Name: Anti-Cathepsin B Antibody [ARC0395]
Brand: Antibodies.com
SKU: A308610
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0395] antibody to Cathepsin B for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Cathepsin B Antibody [ARC0395]

Description

Rabbit monoclonal [ARC0395] antibody to Cathepsin B.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-156 of human Cathepsin B (P07858).

Sequence

SDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0395

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

44 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APP secretase, APPS, Cathepsin B1, CPSB, CTSB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Cathepsin B Antibody [ARC0395] (A308610)

Western blot analysis of extracts of HepG2 cells, using Anti-Cathepsin B Antibody [ARC0395] (A308610) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Immunohistochemistry - Anti-Cathepsin B Antibody [ARC0395] (A308610)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Cathepsin B Antibody [ARC0395] (A308610) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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