Anti-CaSR Antibody (A304836)

Western blot analysis of human HeLa cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Anti-CaSR Antibody (A304836) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 minutes at room temperature. Color Development: TMB. Predicted/Observed Size: 130kDa.

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-CaSR Antibody (A304836), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Multi-Pass membrane Protein. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.