Anti-Caspase-3 Antibody (A83337)

Name: Anti-Caspase-3 Antibody
Brand: Antibodies.com
SKU: A83337
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to Caspase-3 for ELISA, WB and IF/ICC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Caspase-3 Antibody

Description

Goat polyclonal antibody to Caspase-3.

Applications

ELISA, WB, IF/ICC

Dilutions

ELISA: 1:32,000, WB: 0.5-1µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human Caspase-3.

Sequence

C-RDVSKEDHSKRS

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

32 kDa

Predicted MW

31.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Apopain, CASP-3, CASP3, Caspase 3, CPP-32, CPP32, Cysteine protease CPP32, Protein Yama, SCA-1, SREBP cleavage activity 1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in MOLT4 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Caspase-3 Antibody (A83337) at 1µg/ml and detected by chemiluminescence.

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Western Blot - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in Mouse Liver lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Caspase-3 Antibody (A83337) at 0.5µg/ml and detected by chemiluminescence.

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Immunofluorescence - Anti-Caspase-3 Antibody (A83337)

Caspase-3 expression in U251 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Caspase-3 Antibody (A83337) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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