Anti-Caspase-3 Antibody (A80913)

Western blot analysis of extracts of various cell lines, using Anti-Caspase-3 Antibody (A80913) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

Western blot analysis of extracts from normal (control) and Caspase-3 knockout (KO) 293T cells, using Anti-Caspase-3 Antibody (A80913) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-Caspase-3 Antibody (A80913) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using Anti-Caspase-3 Antibody (A80913) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).