Anti-Carbonic Anhydrase IX Antibody [PN-15] - BSA and Azide free (A253535)

Anti-Carbonic Anhydrase IX Antibody [PN-15] - BSA and Azide free

Mouse monoclonal [PN-15] antibody to Carbonic Anhydrase IX.

This antibody recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human, Horse

Microsomal fraction of human renal cortical tissue homogenate.

Mouse

Monoclonal

PN-15

IgG2b

Protein A/G chromatography.

1 mg/ml

55 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Carbonic Anhydrase IX Antibody [PN-15] (A250355).

CA-IX, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250