Recombinant human monoclonal antibody to IL-1 beta.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human IL-1 beta Protein (Fc Tag) (A317954) which can bind Anti-IL-1 beta Antibody [Canakinumab Biosimilar] - Azide free (A318851) in a linear range of 0.61-156.25 µg/ml. In order to specifically detect Anti-IL-1 beta Antibody [Canakinumab Biosimilar] - Azide free (A318851), Mouse Anti-Human IgG Fab Antibody was used as detection antibody.
Flow cytometry analysis under cell membrane permeable condition with Anti-IL-1 beta Antibody [Canakinumab Biosimilar] - Azide free (A318851) at 1 µg/ml on Expi293 cells transfected with Human IL1B protein (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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