Anti-Calretinin Antibody (A104325)

Immunofluorescent analysis of rat cerebellum section stained with Anti-Calretinin Antibody (1:5,000 | red), and co-stained with Anti-Calbindin Antibody (1:1,000 | green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-Calretinin Antibody stains interneurons predominantly in the molecular layer, while the Anti-Calbindin Antibody strongly labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum.

Western blot analysis of different tissue lysates probed with Anti-Calretinin Antibody (1:10,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to the calretinin protein.