Anti-Calretinin Antibody (A104312)

Immunofluorescent analysis of rat hippocampus section stained with Anti-Calretinin Antibody (1:1,000 | green) and co-stained with Anti-Parvalbumin Antibody (1:1,000 | red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Anti-Calretinin Antibody and Anti-Parvalbumin Antibody label different classes of interneurons.

Western blot analysis of different tissue lysates using Anti-Calretinin Antibody (1:1,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to calretinin protein. The same blot was simultaneously probed with Anti-alpha Internexin Antibody (1:10,000 | red) that reveals the alpha internexin protein with apparent molecular weight of 66kDa