Synthetic peptide corresponding to amino acids 191-208 of human Calreticulin. This peptide includes the LIR motif of the molecule, centered on the peptide WxxL, so this antibody may block binding of other molecules to this site.
Sequence
VESGSLEDDWDFLPPKKI
Host
Mouse
Clonality
Monoclonal
Clone ID
6C6
Isotype
IgG1
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
HeLa cell cultures were stained with Anti-Calreticulin Antibody (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with Anti-Vimentin Antibody (A85421 | green). Blue is a DNA stain.
Immunofluorescent analysis of of SH-SY5Y cells stained with Anti-Calreticulin Antibody [6C6] (A85411), at a dilution of 1:500, in green, and co-stained with Anti-Lamin A + C Antibody (A85443), at a dilution of 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The Anti-Calreticulin Antibody [6C6] (A85411) reveals granular staining of cytoplasm, while the Anti-Lamin A + C Antibody (A85443) stains the nuclear lamina and membrane.
Western Blot - Anti-Calreticulin Antibody [6C6] (A85411)
Western blot analysis of lysates of different cell lines using Anti-Calreticulin Antibody [6C6] (A85411), at a dilution of 1:2,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cells, [3] HEK293 cells, [4] HeLa cells, [5] SH-SY5Y cells, [6] C6 cells, and [7] COS-1 cells. A strong single band at about 50 kDa corresponds to the calreticulin protein.
Western Blot - Anti-Calreticulin Antibody [6C6] (A85411)
Western blot analysis of Anti-Calreticulin Antibody in HEK293 (Lane 1), 3T3 (Lane 2), SHSY-5Y (Lane 3) and HeLa cells (Lane 4). The Anti-Calreticulin Antibody binds strongly to calreticulin in 50 kDa.
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