Anti-Calbindin Antibody (A85359)

Name: Anti-Calbindin Antibody
Brand: Antibodies.com
SKU: A85359
Price: 185.0 USD
Availability: InStock

1 Citation

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$185

Chicken polyclonal antibody to Calbindin for WB, ICC/IF and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Tags:

Neuronal Marker

Specifications

Name

Anti-Calbindin Antibody

Description

Chicken polyclonal antibody to Calbindin.

Specificity

This antibody binds Calbindin cleanly but does not cross-react with the related Calretinin and Parvalbumin proteins. It is therefore ideally suited for identifying and subclassifying cortical GABAergic neurons.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:5,000, ICC/IF: 1:1,000-1:5,000, IHC: 1:1,000-1:5,000

Reactivity

Human, Bovine, Rat, Mouse

Immunogen

Recombinant full-length human Calbindin, expressed in and purified from E. coli.

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Conjugate

Unconjugated

Purity

IgY preparation.

Product Form

Liquid

Formulation

Supplied as an aliquot of IgY preparation with 5mM Sodium Azide.

Synonyms

CAB27, CALB1, Calbindin D28, D-28K, Vitamin D-dependent calcium-binding protein, avian-type

Isotype Controls

Chicken IgY (A63673)

Suitable Secondaries

See all Anti-Chicken IgY Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunofluorescence - Anti-Calbindin Antibody (A85359)

Immunofluorescent analysis of rat cerebellum section stained with Anti-Calbindin Antibody (1:2,000 | green) and Anti-MeCP2 Antibody (A85427 | 1:5,000 | red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. Calbindin, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The Anti-MeCP2 Antibody selectively stains nuclei of neuronal cells.

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Immunofluorescence - Anti-Calbindin Antibody (A85359)

Adult rat brain cortex (Left) and striatum (Right) sections (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with Anti-Calbindin Antibody (1:1,000 | red) and Anti-Fox3/NeuN Antibody (A85405 | green). Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express Fox3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is DAPI nucleus staining.

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Western Blot - Anti-Calbindin Antibody (A85359)

Western blot analysis of different tissue lysates and recombinant protein solutions using Anti-Calbindin Antibody (1:5,000 | green): [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins: [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. The Anti-Calbindin Antibody specifically recognises calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

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Western Blot - Anti-Calbindin Antibody (A85359)

Western blot analysis of Anti-Calbindin Antibody. Blots of rat brain lysate (Left) and 0.5 µg of His-tagged recombinant proteins (Right) were probed with Anti-Calbindin Antibody (1:5,000): Lane1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin. In rat brain lysates, this antibody recognizes a clear band at 30 kDa which represents calbindin and it reacts only with calbindin protein, and not other calcium-binding proteins. The band at ~60 kDa is most likely the dimer of calbindin.

Product Citations (1)

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Non-photoreceptor Expression of Tulp1 May Contribute to Extensive Retinal Degeneration in Tulp1-/- Mice

This publication cites Anti-Calbindin Antibody (A85359).

Article Abstract

Mutations in tubby like protein 1 gene (TULP1) are causative of early-onset recessive inherited retinal degenerations (IRDs); similarly, the Tulp1-/- mouse is also characterized by a rapid IRD. Tulp1 mRNA and protein expression was analyzed in wild type mouse retinas and expression data sets (NCBI) during early postnatal development. Comparative histology was undertaken in Tulp1-/-, rhodopsin-/- (Rho-/-) and retinal degeneration slow-/- (Rds-/-) mouse retinas. Bioinformatic analysis of predicted TULP1 interactors and IRD genes was performed. Peak expression of Tulp1 in healthy mouse retinas was detected at p8; of note, TULP1 was detected in both the outer and inner retina. Bioinformatic analysis indicated Tulp1 expression in retinal progenitor, photoreceptor and non-photoreceptor cells. While common features of photoreceptor degeneration were detected in Tulp1-/-, Rho-/-, and Rds-/- retinas, other alterations in bipolar, amacrine and ganglion cells were specific to Tulp1-/- mice. Additionally, predicted TULP1 interactors differed in various retinal cell types and new functions for TULP1 were suggested. A pilot bioinformatic analysis indicated that in a similar fashion to Tulp1, many other IRD genes were expressed in both inner and outer retinal cells at p4-p7. Our data indicate that expression of Tulp1 extends to multiple retinal cell types; lack of TULP1 may lead to primary degeneration not only of photoreceptor but also non-photoreceptor cells. Predicted interactors suggest widespread retinal functions for TULP1. Early and widespread expression of TULP1 and some other IRD genes in both the inner and outer retina highlights potential hurdles in the development of treatments for these IRDs.