Anti-C5a-R Antibody [20/70] (PE) (A279123)

Anti-C5a-R Antibody [20/70] (PE)

Rat monoclonal [20/70] antibody to C5a-R (PE).

This antibody recognises mouse CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45 kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88,.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Mouse

RBL-2H3 transfected cells expressing mouse C5aR.

Rat

Monoclonal

20/70

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD88 antibody, clone 20/70 recognizes murine CD88, also known as C5a anaphylatoxin chemotactic receptor 1 (C5aR1), a member of the G-protein coupled receptor 1 family. CD88 is a ~45 kDa multi pass membrane protein and functions as a receptor for the complement component C5a, a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Rat anti Mouse CD88 antibody, clone 20/70 is able to block the binding of the C5a to murine CD88, (Soruri et al. 2003).

C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5AR1, C5R1, CD88

This product is for research use only. It is not intended for diagnostic or therapeutic use.