Anti-C5a-R Antibody [10/92] (Biotin) (A278840)

Anti-C5a-R Antibody [10/92] (Biotin)

Rat monoclonal [10/92] antibody to C5a-R (Biotin).

This antibody recognises mouse CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B/C).

Mouse

RBL-2H3 transfected cells expressing mouse C5aR.

Rat

Monoclonal

10/92

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88 (Souri et al. 2003).

C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor 1, C5AR1, C5R1, CD88

This product is for research use only. It is not intended for diagnostic or therapeutic use.