Anti-C4b Antibody (A329166)

Name: Anti-C4b Antibody
Brand: Antibodies.com
SKU: A329166
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to C4b for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-C4b Antibody

Description

Rabbit polyclonal antibody to C4b.

Applications

WB, ICC/IF, ELISA

Positive Control

Hep G2, Mouse liver, Mouse spleen, Rat liver, Rat thymus.

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1337-1446 of human C4b.

Sequence

NGFKSHALQLNNRQIRGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYTMEANEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

85 kDa

Predicted MW

192 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Acidic complement C4, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, C4A, CO4, Complement C4-A, Complement C4-B, CPAMD2, CPAMD3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-C4b Antibody (A329166)

C4b expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-C4b Antibody (A329166) at a dilution of 1:2000 and detected with chemiluminescence.

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Immunofluorescence - Anti-C4b Antibody (A329166)

C4b expression in HepG2 cells analyzed by immunofluorescence. Staining was performed with Anti-C4b Antibody (A329166) at a dilution of 1:200 followed by Cy3 Goat Anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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Immunofluorescence - Anti-C4b Antibody (A329166)

C4b expression in Mouse liver tissue analyzed by immunofluorescence. Staining was performed with Anti-C4b Antibody (A329166) at a dilution of 1:200 followed by Cy3 Goat Anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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