Anti-C3 Antibody [AB01-3B4] (A279621)

Anti-C3 Antibody [AB01-3B4]

Mouse monoclonal [AB01-3B4] antibody to C3.

This antibody recognises complement C3. C3 is a central component of the classical, alternate and lectin pathways in the complement cascade which forms part of the innate immune defenses. The central position of C3 in the cascade has led to the emergence of immune invasion strategies by many human pathogens, including Staphylococcus aureus which expresses 2 proteins that interfere with the activation of C3. C3 Deficiencies in humans also show an increased susceptibility to bacterial infections in early childhood the development of autoimmune disease and kidney diseases. Conversely, complement over-activation can result in various disease pathologies, including induction of inflammatory pathways causing tissue damage. With complements’ role in various diseases, pharmaceutical and biotech companies have targeted the cascade for potential therapeutic targets in a range of immune and inflammatory diseases as well as in transplantation medicine. C3 is activated via its’ cleavage into fragments which have varying functions within the complement cascade.

WB: 1:1,000, Under reducing conditions the C3 alpha chain at an approximate molecular weight of 115 kDa and the C3c alpha chain fragment 2 at approximately 39 kDa can be detected by WB. Under non reducing conditions the mature C3 band can be detected at a molecular weight of approximately 186 kDa

Human

E. coli derived recombinant human C3 (Gly1200-Asn1663).

GRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN

Mouse

Monoclonal

AB01-3B4

IgG1

Protein G affinity chromatography.

1 mg/ml

Approximately 115 kDa and 39 kDa corresponding to the C3 alpha chain and the C3c alpha chain fragment 2 under reducing conditions. Under non-reducing conditions the mature C3 band can be detected at approximately 186 kDa.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human C3 antibody, clone AB01-384 recognizes complement C3. C3 is a central component of the classical, alternate and lectin pathways in the complement cascade which forms part of the innate immune defenses. The central position of C3 in the cascade has led to the emergence of immune invasion strategies by many human pathogens, including Staphylococcus aureus which expresses 2 proteins that interfere with the activation of C3 (Garcia et al. 2012). C3 Deficiencies in humans also show an increased susceptibility to bacterial infections in early childhood the development of autoimmune disease and kidney diseases (Ricklin et al. 2016). Conversely, complement over-activation can result in various disease pathologies, including induction of inflammatory pathways causing tissue damage. With complements’ role in various diseases, pharmaceutical and biotech companies have targeted the cascade for potential therapeutic targets in a range of immune and inflammatory diseases as well as in transplantation medicine (Ricklin et al. 2012). C3 is activated via its’ cleavage into fragments which have varying functions within the complement cascade.

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, Complement C3, CPAMD1