Anti-c-Myc (phospho Ser62) Antibody (A12455)

Name: Anti-c-Myc (phospho Ser62) Antibody
Brand: Antibodies.com
SKU: A12455
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to c-Myc (phospho Ser62) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Epitope Tag Hematopoietic Stem Cell Marker

Specifications

Name

Anti-c-Myc (phospho Ser62) Antibody

Description

Rabbit polyclonal antibody to c-Myc (phospho Ser62).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S62 of human MYC.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

BHLHE39, Class E basic helix-loop-helix protein 39, MYC, Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-c-Myc (phospho Ser62) Antibody (A12455)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-c-Myc (phospho Ser62) Antibody (A12455) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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