Anti-c-Myc Antibody [9E10] (A278887)

Anti-c-Myc Antibody [9E10]

Mouse monoclonal [9E10] antibody to c-Myc.

This antibody recognises the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence.

WB, IHC-P, IHC-Fr, ELISA, Flow Cytometry

ELISA: 1:100 - 1:500, FC 1: Neat for 20 µg, 0.1 mg, 1 mg, 1:10 for 2 mg - 1:10 for 20 µg, 0.1 mg, 1 mg, 1:50 for 2 mg, WB 2: 1:100 - 1:500, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human, Epitope tag

Synthetic peptide corresponding to amino acids 408-439 from the C-terminus of human c-myc conjugated to Keyhole Limpet Haemocyanin.

VVILKKATAYILSVQAEEQKLISEEDLLRKRR

Mouse

Monoclonal

9E10

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).

BHLHE39, Class E basic helix-loop-helix protein 39, MYC, Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64