Western Blot - Anti-c-Kit (phospho Tyr719) Antibody (A11064)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-c-Kit (phospho Tyr719) Antibody (A11064) at 1:500 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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