Recombinant chimeric monoclonal [DMC446] antibody to c-Kit.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
DMC446
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Flow cytometry analysis of Anti-c-Kit Antibody [DMC446] - BSA and Azide free (A318732) on Expi293 cells transfected with human CD117 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Flow cytometry analysis of antigen binding of Anti-c-Kit Antibody [DMC446] - BSA and Azide free (A318732). (A) Anti-c-Kit Antibody [DMC446] - BSA and Azide free (A318732) does not bind to A431 cells that do not express CD117. (B) A clear peak shift of Anti-c-Kit Antibody [DMC446] - BSA and Azide free (A318732) was seen compared to the control when incubated with CD117-expressing TT cells, indicating strong binding of Anti-c-Kit Antibody [DMC446] - BSA and Azide free (A318732) to CD117. Antibodies were incubated at 5 µg/ml. .
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