Anti-c-Kit Antibody (A8390)

Name: Anti-c-Kit Antibody
Brand: Antibodies.com
SKU: A8390
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to c-Kit for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Embryonic Stem Cell Marker Endothelial Stem Cell Marker Hematopoietic Stem Cell Marker Mesenchymal Stem Cell Marker

Specifications

Name

Anti-c-Kit Antibody

Description

Rabbit polyclonal antibody to c-Kit.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 875-976 of human CD117/c-Kit (NP_000213.1).

Sequence

VDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPNRQKPVVDHSVRINSVGSTASSSQPLLVHDDV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

145 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD117, KIT, Mast/stem cell growth factor receptor Kit, p145 c-kit, PBT, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-c-Kit Antibody (A8390)

Western blot analysis of Mouse lung, using Anti-c-Kit Antibody (A8390) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Immunohistochemistry - Anti-c-Kit Antibody (A8390)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-c-Kit Antibody (A8390) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Kit Antibody (A8390)

Immunohistochemistry analysis of paraffin-embedded mouse intestin using Anti-c-Kit Antibody (A8390) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Kit Antibody (A8390)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-c-Kit Antibody (A8390) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.