Anti-c-Kit Antibody [2B8/BM] (A279192)

Anti-c-Kit Antibody [2B8/BM]

Mouse monoclonal [2B8/BM] antibody to c-Kit.

This antibody recognises CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.

WB, IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10 - 1:20, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Porcine

Porcine bone marrow cells (BMC).

Mouse

Monoclonal

2B8/BM

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD117, clone 2B8/BM is specific for porcine CD117, also known as c-kit, a 155 kDa type I transmembrane protein with protein tyrosine kinase activity, which plays an important role in early hematopoiesis (Perez et al. 2007). Hematopoietic stem cells (HSC) of bone marrow have multilineage differentiation potential and an extensive capacity for self-renewal. The majority of adult bone marrow hematopoietic progenitor cells are CD117+ and have been used successfully in xenograft transplantation models for long term survival of grafts, without symptoms of graft-versus-host disease (GVHD). Mouse anti pig CD117, clone 2B8/BM recognizes CD117 on a small subset of porcine bone marrow progenitor cells and therefore provides an alternative tool from the previously used c-kit ligand stem cell factor, for the isolation and enrichment of porcine stem cells.

CD117, KIT, Mast/stem cell growth factor receptor Kit, p145 c-kit, PBT, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog