This antibody recognises mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.recognises an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Mouse
Immunogen
IL-3 dependent mast cells.
Host
Rat
Clonality
Monoclonal
Clone ID
2B8
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse CD117 antibody, clone 2B8 recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.