Anti-c-Jun (phospho Thr91) Antibody (A308991)

Name: Anti-c-Jun (phospho Thr91) Antibody
Brand: Antibodies.com
SKU: A308991
Price: 450.0 USD
Availability: InStock

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$450

Rabbit polyclonal antibody to c-Jun (phospho Thr91) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-c-Jun (phospho Thr91) Antibody

Description

Rabbit polyclonal antibody to c-Jun (phospho Thr91).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T91 of human JUN (NP_002219.1).

Sequence

TTTPT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activator protein 1, AP1, JUN, p39, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, Transcription factor Jun, V-jun avian sarcoma virus 17 oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-c-Jun (phospho Thr91) Antibody (A308991)

Western blot analysis of extracts of various cell lines, using Anti-c-Jun (phospho Thr91) Antibody (A308991) at 1:1,000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-c-Jun (phospho Thr91) Antibody (A308991)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-c-Jun (phospho Thr91) Antibody (A308991) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Jun (phospho Thr91) Antibody (A308991)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-c-Jun (phospho Thr91) Antibody (A308991) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Jun (phospho Thr91) Antibody (A308991)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-c-Jun (phospho Thr91) Antibody (A308991) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.