Anti-c-Jun (phospho Ser63) Antibody (A306757)

Western blot analysis of extracts of 293T cells, using Anti-c-Jun (phospho Ser63) Antibody (A306757) at 1:1,000 dilution. 293T cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.