Anti-c-Jun (phospho Ser63) Antibody (A306757)

Name: Anti-c-Jun (phospho Ser63) Antibody
Brand: Antibodies.com
SKU: A306757
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to c-Jun (phospho Ser63) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-c-Jun (phospho Ser63) Antibody

Description

Rabbit polyclonal antibody to c-Jun (phospho Ser63).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S63 of human Jun (NP_002219.1).

Sequence

LTSPD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

44 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Activator protein 1, AP1, JUN, p39, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, Transcription factor Jun, V-jun avian sarcoma virus 17 oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-c-Jun (phospho Ser63) Antibody (A306757)

Western blot analysis of extracts of 293T cells, using Anti-c-Jun (phospho Ser63) Antibody (A306757) at 1:1,000 dilution. 293T cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-c-Jun (phospho Ser63) Antibody (A306757)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Jun (phospho Ser63) Antibody (A306757)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-c-Jun (phospho Ser63) Antibody (A306757)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-c-Jun (phospho Ser63) Antibody (A306757) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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