Western blot analysis of extracts of HeLa cells, using Anti-c-Fos Antibody (A91695) at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Publishing research using Anti-c-Fos Antibody (A91695)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-c-Fos Antibody (A91695)