Western blot analysis of extracts of HeLa cells, using Anti-c-Fos Antibody (A12562) at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-c-Fos Antibody (A12562). This Western blot was performed on the immunoprecipitate using Anti-c-Fos Antibody (A12562) at a dilution of 1:1000.
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