Anti-c-Fos Antibody [5B10] (A305156)

Western blot analysis of human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Anti-c-Fos Antibody [5B10] (A305156) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

Immunohistochemistry analysis of rat brain. The Primary Antibody used was Anti-c-Fos Antibody [5B10] (A305156) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 10X.