This antibody recognizes an epitope within the C-terminal region of human BRG1; a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
Applications
WB
Dilutions
WB: 10 µg/ml; Positive Control: HEK293 cells.
Reactivity
Human
Immunogen
Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
Host
Mouse
Clonality
Monoclonal
Clone ID
BRG-01
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
205 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SMARCA4, SNF2B, SNF2L4, Transcription activator BRG1
Western Blot - Anti-BRG1 Antibody [BRG-01] (A86541)
Immunostaining of Brg1 by Anti-Brg1 Antibody (A86541). A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression. B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.
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