Western blot analysis of extracts from normal (control) and BRG1/BRG1/SMARCA4 knockout (KO) 293T cells, using Anti-BRG1 Antibody (A13884) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-BRG1 Antibody (A13884) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-BRG1 Antibody (A13884) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-BRG1 Antibody (A13884). This Western blot was performed on the immunoprecipitate using Anti-BRG1 Antibody (A13884) at a dilution of 1:1000.
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