Anti-BrdU Antibody [YR-BrdU] (A58171)

Anti-BrdU Antibody [YR-BrdU]

Mouse monoclonal [YR-BrdU] antibody to BrdU.

IHC, Flow Cytometry

Dependending on Assay: 1:500-1:2,000.

5-iodouridine covalently coupled to KLH.

Mouse

Monoclonal

YR-BrdU

IgG1

Protein A chromatography.

Lyophilized

Reconstitute with sterile water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Lyophilized from Phosphate Buffered Saline.

Shipped at 4°C. Lyophilized: Long term store at 2-8°C in a dry environment. Reconstituted: Aliquot and store at -20°C long term or use within a month at 4°C. Avoid freeze/thaw cycles.

This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.