Anti-Brd4 (phospho Thr204) Antibody (A305447)

Name: Anti-Brd4 (phospho Thr204) Antibody
Brand: Antibodies.com
SKU: A305447
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Brd4 (phospho Thr204) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Brd4 (phospho Thr204) Antibody

Description

Rabbit polyclonal antibody to Brd4 (phospho Thr204).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1).

Sequence

ASTPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Bromodomain-containing protein 4, HUNK1, Protein HUNK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Brd4 (phospho Thr204) Antibody (A305447)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Brd4 (phospho Thr204) Antibody (A305447) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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