Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01] (A306225)

Name: Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01]
Brand: Antibodies.com
SKU: A306225
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC5019-01] antibody to Brd4 (phospho Ser1083) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01]

Description

Rabbit monoclonal [ARC5019-01] antibody to Brd4 (phospho Ser1083).

Applications

Dilutions

WB: 1:100-1:500

Reactivity

Human

Immunogen

A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1).

Sequence

HQSPP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5019-01

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Bromodomain-containing protein 4, HUNK1, Protein HUNK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01] (A306225)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01] (A306225) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 300s.

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