Rabbit polyclonal antibody to BRCA1 (Phospho-Ser1457)
Specificity
The antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1457.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 1457 (L-T-S(p)-Q-K) derived from Human BRCA1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53
Western blot analysis of extracts from 293 cells (Lane 2), using BRCA1 (Phospho-Ser1457) Antibody #11787. The lane on the left is treated with antigen-specific peptide.
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