Rabbit polyclonal antibody to BRCA1 (Phospho-Ser1423)
Specificity
The antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.
Applications
WB, IHC
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 1423 (H-G-S(p)-Q-P) derived from Human BRCA1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Phospho-Ser1423) Antibody #11242 (left) or the same antibody preincubated with blocking peptide (right).
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