Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Name: Anti-BRCA1 (phospho Ser1423) Antibody
Brand: Antibodies.com
SKU: A10901
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to BRCA1 (phospho Ser1423) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-BRCA1 (phospho Ser1423) Antibody

Description

Rabbit polyclonal antibody to BRCA1 (phospho Ser1423).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).

Sequence

HGSQP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

270 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Western blot analysis of extracts of HT-29 cells, using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at 1:2,000 dilution. HT-29 cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BRCA1 (phospho Ser1423) Antibody (A10901)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-BRCA1 (phospho Ser1423) Antibody (A10901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.