Anti-BRCA1 Antibody [MS13] (A281287)

Anti-BRCA1 Antibody [MS13]

Mouse monoclonal [MS13] antibody to BRCA1.

This antibody recognises the human tumor suppressor protein BRCA-1, also known as Breast cancer type 1 susceptibility protein or RING finger protein 53. BRCA1 is a 1863 amino acid, ~220 kDa E3 ubiquitin-protein ligase playing a central role in DNA repair , expressed in the nucleus during the S/G2 phase of the cell cycle. Normal BRCA-1 acts as a tumor suppressor protein and mutation or dysregulation of BRCA1 may indicate high risk of development of disease, including breast cancer. recognises an epitope within the 304 amino acid N-terminal region of human BRCA1.BRCA1 is expressed numerous organs including mammary and ovarian tissues. Mutations in the BRCA-1 gene are associated with hereditary breast and ovarian cancers. Secondary cancers such as prostate or melanoma also frequently arise in the latter stages of both male and female breast cancer patients , but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly. However, in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations. BRCA1 is a key marker of triple-negative breast cancer (TNBC), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and lacks the benefit of specific targeted therapy. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Mouse anti human BRCA1 antibody, clone MS13 is suitable for use in the immunohistochemical staining of human tissues and for immunofluorescence in multiple cell lines.

WB, IHC-P, IF, IP

IF: 1:500, IHC-P: 1:50, WB: 0.5 µg/ml

Human

Recombinant protein corresponing to the N-terminus of human BRCA1.

Mouse

Monoclonal

MS13

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Approximately 220 kDa in HeLa nuclear extract. Some cell lysates may also show an uncharacterized band of approximately 65 kDa.

Liquid

Supplied in TRIS-glycine Buffered Saline with NaCl and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human BRCA1 antibody, clone MS13 recognizes the human tumor suppressor protein BRCA-1, also known as Breast cancer type 1 susceptibility protein or RING finger protein 53. BRCA1 is a 1863 amino acid, ~220 kDa E3 ubiquitin-protein ligase playing a central role in DNA repair (Tibbetts et al. 2000), expressed in the nucleus during the S/G2 phase of the cell cycle (Durrant and Nickoloff 2005). Normal BRCA-1 acts as a tumor suppressor protein and mutation or dysregulation of BRCA1 may indicate high risk of development of disease, including breast cancer (Budhram-Mahadeo et al. 1999). Mouse anti human BRCA-1, clone MS13 recognizes an epitope within the 304 amino acid N-Terminal (NT) region (Yoshikawa et al. 1999) of human BRCA1.BRCA1 is expressed numerous organs including mammary and ovarian tissues (Miki et al. 1994) . Mutations in the BRCA-1 gene are associated with hereditary breast and ovarian cancers. Secondary cancers such as prostate or melanoma also frequently arise in the latter stages of both male and female breast cancer patients (Benevento et al. 2012), but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly (Scheuer et al. 2002). However, in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations (Gallagher et al. 2010).BRCA1 is a key marker of triple-negative breast cancer (TNBC), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and lacks the benefit of specific targeted therapy (Duffy et al. 2012). Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Mouse anti human BRCA1 antibody, clone MS13 is suitable for use in the immunohistochemical staining of human tissues (Fraser et al. 2003) and for immunofluorescence in multiple cell lines (Scully et al. 1996).

Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53