Anti-BRCA1 Antibody [MS110] (A281286)

Anti-BRCA1 Antibody [MS110]

Mouse monoclonal [MS110] antibody to BRCA1.

This antibody recognises an epitope within the 304 amino acid N-terminal region of human BRCA1, otherwise known as Breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.Deleterious BRCA1 mutations corralate with increased risk of cancers in both males and females including breast, pancreatic and prostate cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women. Mouse anti human BRCA1 antibody, clone MS110 is suitable for use in both immunohistochemical and immunofluorescence staining of human breast and ovarian tissue, and clone MS110 is widely used and reported (Milner et al. 2013).

WB, IHC-P, IF, Flow Cytometry, IHC-Fr, IP

WB: 1 µg/ml

Human

Recombinant protein corresponding to the N-terminus of human BRCA1.

Mouse

Monoclonal

MS110

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Approximately 220 kDa in HeLa nuclear extract. A smaller band of 85 kDa may also be seen, which may represent post-translational modification.

Liquid

Supplied in TRIS-glycine Buffered Saline with NaCl and <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human BRCA1 antibody, clone MS110 recognizes an epitope within the 304 amino acid N-Terminal (NT) region of human BRCA1, otherwise known as Breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.Deleterious BRCA1 mutations corralate with increased risk of cancers in both males and females including breast, pancreatic and prostate cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women. Mouse anti human BRCA1 antibody, clone MS110 is suitable for use in both immunohistochemical and immunofluorescence staining of human breast and ovarian tissue, and clone MS110 is widely used and reported (Milner et al. 2013).

Breast cancer type 1 susceptibility protein, RING finger protein 53, RING-type E3 ubiquitin transferase BRCA1, RNF53