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Anti-Bovine IgG2 Antibody [IL-A73] (HRP) (A280735)

This product is discontinued

Anti-Bovine IgG2 Antibody [IL-A73] (HRP) (A280735) has been discontinued and is no longer available.

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Name
Anti-Bovine IgG2 Antibody [IL-A73] (HRP)
Description
Mouse monoclonal [IL-A73] antibody to Bovine IgG2 (HRP).
Specificity
This antibody recognises bovine IgG2 and does not recognise other bovine immunoglobulin classes.While normal serum levels of IgG1 are relatively constant in cattle at around 50% , IgG2 levels are highly variable between different strains and races. Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2.
Applications
ELISA
Dilutions
ELISA: 1:100 - 1:1,000
Reactivity
Bovine
Immunogen
Purified bovine IgG2.
Host
Mouse
Clonality
Monoclonal
Clone ID
IL-A73
Isotype
IgG2a
Conjugate

HRP

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and HRP Stabiliser (Proprietary stabiliser based on a TRIS buffer, <15ppm CMIT/MIT).
Storage
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine IgG2, clone IL-A73, is a monoclonal antibody specific for bovine IgG2 and does not recognise other bovine immunoglobulin classes.While normal serum levels of IgG1 are relatively constant in cattle at around 50%, IgG2 levels are highly variable between different strains and races. Mouse anti Bovine IgG2, clone IL-A73 immunoprecipitates a protein band of approximately 52-59kDa, consistent with the heavy chain of bovine IgG2 (Campbell et al. 1998).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.