Anti-BOB1 Antibody (A83883)

Name: Anti-BOB1 Antibody
Brand: Antibodies.com
SKU: A83883
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to BOB1 for ELISA and WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Specifications

Name

Anti-BOB1 Antibody

Description

Goat polyclonal antibody to BOB1.

Applications

ELISA, WB

Dilutions

ELISA: 1:8,000, WB: 1-3µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human BOB1.

Sequence

C-DAYALNHTLSVEGF

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30 kDa

Predicted MW

28kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

B-cell-specific coactivator OBF-1, BOB-1, OBF1, OCA-B, OCT-binding factor 1, POU domain class 2-associating factor 1, POU2AF1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-BOB1 Antibody (A83883)

BOB1 expression in Human (A), Mouse (B), and Rat (C) Spleen cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-BOB1 Antibody (A83883) at 0.3µg/ml and detected by chemiluminescence.

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Western Blot - Anti-BOB1 Antibody (A83883)

BOB1 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress FLAG tagged BOB1 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-BOB1 Antibody (A83883) at 1µg/ml (Lane A) or Anti-FLAG at 1:3000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-BOB1 Antibody (A83883) at 1mg/ml (Lane B).

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