Anti-Bmi1 Antibody (A85202)

Name: Anti-Bmi1 Antibody
Brand: Antibodies.com
SKU: A85202
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to Bmi1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-Bmi1 Antibody

Description

Goat polyclonal antibody to Bmi1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:128,000, WB: 1-3µg/ml, IHC: 5µg/ml

Reactivity

Human, Mouse, Porcine

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 237-251, of human Bmi1.

Sequence

C-SHQRDGLTNAGELES

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

40 kDa

Predicted MW

36.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, RNF51

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Bmi1 Antibody (A85202)

Bmi1 expression in K562 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Bmi1 Antibody (A85202) at 0.1µg/ml and detected by chemiluminescence.

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Western Blot - Anti-Bmi1 Antibody (A85202)

Bmi1 expression in NIH3T3 nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Bmi1 Antibody (A85202) at 0.3µg/ml and detected by chemiluminescence.

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Western Blot - Anti-Bmi1 Antibody (A85202)

Bmi1 expression in Pig Bone Marrow lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Bmi1 Antibody (A85202) at 2µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-Bmi1 Antibody (A85202)

Bmi1 expression in Human Testis analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Bmi1 Antibody (A85202) at 5µg/ml and revealed with alkaline phosphatase (AP).

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