IgG4PE - Human IgG4 with S228P and L235E mutations
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 150 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Bleselumab Biosimilar - Anti-CD40 Antibody - Low endotoxin, Azide free (A323325) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Bleselumab Biosimilar - Anti-CD40 Antibody - Low endotoxin, Azide free (A323325) confirms that the purity of this antibody is 99.21%.
Human CD40 CHO cells were stained with Bleselumab Biosimilar - Anti-CD40 Antibody - Low endotoxin, Azide free (A323325) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.6311 µg/ml.
Bleselumab Biosimilar - Anti-CD40 Antibody - Low endotoxin, Azide free (A323325) FACS blocking was evaluated using CCRF-CEM. The IC50 was approximately 1.359 µg/ml.
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