Western blot analysis of human lung carcinoma epithelial cell line (A549) lysate showing detection of BIM protein using Anti-Bim Antibody (A304982) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.