Synthetic peptide corresponding to the internal region of human Bile Acid Receptor NR1H4.
Sequence
KSCREKTELTPDQQ
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
55 kDa
Predicted MW
54.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
BAR, Bile acid receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1, FXR, HRR1, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14, RIP14, RXR-interacting protein 14
Bile Acid Receptor NR1H4 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Bile Acid Receptor NR1H4 Antibody (A84276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Bile Acid Receptor NR1H4 expression in U2OS cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Bile Acid Receptor NR1H4 Antibody (A84276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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