Anti-beta III Tubulin Antibody [TU-20] (A86691)

Anti-beta III Tubulin Antibody [TU-20]

Mouse monoclonal [TU-20] antibody to beta III Tubulin.

The antibody recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Flow Cytometry (Intracellular), WB, IHC-P, ICC

WB: 1-2 µg/ml; Positive Control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; Reducing conditions, IHC-P: 10 µg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; Positive Control: neuronal tissue, IHC: Positive Control: Neuro2a mouse neuroblastoma cell line, Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Canine, Mouse, Rat, Porcine, Human

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Mouse

Monoclonal

TU-20

Protein A chromatography.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III